Proteases and Cancer

dissecting Degradomes: Analysis of Protease-Coding Genes.- Identification of Protease Cleavage Sites and Substrates in Cancer by Carboxy-TAILS (C-TAILS).- Identification of Proteolytic Cleavage Sites of EphA2 by Membrane Type-1 Matrix Metalloproteinase on the Surface of Cancer Cells.- Biotin Chasing Assay to Evaluate uPAR Stability and Cleavage on the Surface of Cells.- Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease.- A Simple Cell-Based Assay for the Detection of Surface Protein Shedding by Rhomboid Proteases.- Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm.- Autophagy and Proteases: Basic Study of the Autophagic Flux by Western Blot.- Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture.- Analysis of Enzymatic Activity of Matrix Metalloproteinase (MMP) by Collagen Zymography in Melanoma.- Measurement of Protease Activities using Fluorogenic Substrates.- Targeting theExpression of Cathepsin B using CRISPR/Cas9 System in Mammalian Cancer Cells.- Assessing the Influence of a Protease in Cell Migration using the Barrier-Migration Assay.- Analysis of Invasive Activity of CAF-Spheroids into Three-Dimensional (3D) Collagen Matrices.- Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains using Tri-Dimensional (3D) Spheroid Invasion Assay.- Analyzing the Type II Transmembrane Serine Protease Hepsin-Dependent Basement Membrane Remodeling in 3D Cell Culture.- Evaluation of Tumor Vasculature using a Syngeneic Tumor Model in Wild Type and Genetically Modified Mice.- 3D Image Analysis of the Microvasculature in Healthy and Diseased Tissues.- Human Tumor Tissue-Based 3D In Vitro Invasion Assays.- Ear Sponge Assay: A Method to Investigate Angiogenesis and Lymphangiogenesis in Mice.- Cancer Susceptibility Models in Protease-Deficient Mice.- Imaging Proteolytic Activities in Mouse Models of Cancer.- Protease Silencing to Explore the Molecular Mechanisms of Cancer and Aging.- Analysis of Somatic DNA Methylation Alterations of Genes Encoding Cell Surface Metallopeptidases in Colorectal Cancer.- Targeting Deubiquitinases in Cancer.- Generation of Highly Selective MMP Antibody Inhibitors.- Strategies to Target Matrix Metalloproteinases as Therapeutic Approach in Cancer.