Leishmania

Host-Free Systems for Differentiation of Axenic Leishmania.- Phylogenetic Studies.- A Guide to Next Generation Sequence Analysis of Leishmania Genomes.- Quantitative RNA Analysis using RNA Seq.- Ribosome Profiling in Trypanosomatids.- Cosmid Library Construction and Functional Cloning.- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania.- Gene Replacement by Homologous Recombination.- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9.- DiCre-Based Inducible Disruption of Leishmania Genes.- DiCre-Based Inducible Gene Expression.- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments.- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR).- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening.- Quantification of Parasite Loads by Automated Microscopic Image Analysis.- Quantification of Leishmania Parasites in Murine Models of Visceral Infection.- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis.- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease.- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.