RNA Methylation

LC-MS Analysis of Methylated RNA.- Comparative Analysis of Ribonucleic Acid Digests (CARD) by mass Spectrometry.- Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation.- Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-seq).- Mapping m6A at Individual-Nucleotide Resolution using Crosslinking and Immunoprecipitation (miCLIP).- Detection and Quantification of N6-Methyladenosine in Messenger RNA by TLC.- Illustrating the Epitranscriptome at Nucleotide Resolution using Methylation-iCLIP (miCLIP).- Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing.- Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing.- Analysis of High-Throughput RNA Bisulfite Sequencing Data.- Statistcal Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite.- High-Throughput Mapping of 2’-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol).- RiboMeth-seq: Profiling of 2’-O-Me in RNA.- In Silico Identification of RNA Modifications from High-Throughput Sequencing Data using HAMR.- High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-seq).- Transcriptome-Wide Mapping of N1-Methyladenosine Methylome.- In Vitro Assays for RNA Methyltransferase Activity.- Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo.- Methylated mRNA Nucleotides as Regulators for Ribosomal Translation.- Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.