High-Resolution Imaging of Cellular Proteins
Humana Press Inc. (Verlag)
978-1-4939-8173-1 (ISBN)
Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture.- Antibody Production with Synthetic Peptides.- Production and Purification of Polyclonal Antibodies.- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab’)2 and Streptavidin.- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications.- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells.- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells.- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.- Imaging SynapticVesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins.- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas.- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues.- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae.- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.- Pre-Embedding Nanogold Silver and Gold Intensification.- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval.- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections.- Immuno-Gold Labelling for Scanning Electron Microscopy.- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope.- Functional Nanoscale Imaging Of Synaptic VesicleCycling With Superfast Fixation.
Erscheinungsdatum | 25.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1474 |
Zusatzinfo | 21 Illustrations, color; 24 Illustrations, black and white; XIII, 366 p. 45 illus., 21 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizinische Fachgebiete ► Radiologie / Bildgebende Verfahren ► Radiologie |
Schlagworte | anti-peptide antibodies • conventional fluorophores • cryo-ultramicrotomy • epitope tagged proteins • fluorescent microscopy toolbox • molecular tool box • polyclonal antibodies • rapid freeze-replacement fixation |
ISBN-10 | 1-4939-8173-0 / 1493981730 |
ISBN-13 | 978-1-4939-8173-1 / 9781493981731 |
Zustand | Neuware |
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