Single Nucleotide Polymorphisms -

Single Nucleotide Polymorphisms

Methods and Protocols

Pui-Yan Kwok (Herausgeber)

Buch | Hardcover
288 Seiten
2002
Humana Press Inc. (Verlag)
978-0-89603-968-1 (ISBN)
90,90 inkl. MwSt
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Provides reproducible techniques for the process of single nucleotide polymorphisms (SNP) discovery and genotyping. This book offers methods for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

SNPs: Why Do We Care?Lisa D. BrooksDenaturing High-Performance Liquid ChromatographyAndreas Premstaller and Peter J. OefnerSNP Detection and Allele Frequency Determination by SSCPTomoko Tahira, Akari Suzuki, Yoji Kukita, and Kenshi HayashiConformation-Sensitive Gel ElectrophoresisArupa GangulyDetection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified AssayChinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, and Richard G. H. CottonSNP Discovery by Direct DNA SequencingPui-Yan Kwok and Shenghui DuanComputational SNP Discovery in DNA Sequence DataGabor T. MarthGenotyping SNPs With Molecular BeaconsSalvatore A. E. Marras, Fred Russell Kramer, and Sanjay TyagiSNP Genotyping by the 5'-Nuclease ReactionKenneth J. LivakGenotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide MicroarraysKatarina Lindroos, Ulrika Liljedahl, and Ann-Christine SyvanenQuantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase MinisequencingCharlotta Olsson, Ulrika Liljedahl, and Ann-Christine SyvanenHomogeneous Primer Extension Assay With Fluorescence Polarization DetectionTony M. Hsu and Pui-Yan KwokPyrosequencing for SNP GenotypingMostafa RonaghiHomogeneous Allele-Specific PCR in SNP GenotypingSoren Germer and Russell HiguchiOligonucleotide Ligation AssayJonas Jarvius, Mats Nilsson, and Ulf LandegrenInvader Assay for SNP GenotypingVictor Lyamichev and Bruce NeriMALDI-TOF Mass Spectrometry-Based SNP GenotypingNiels Storm, Brigitte Darnhofer-Patel, Dirk van den Boom, and Charles P. RodiIndex

Erscheint lt. Verlag 7.10.2002
Reihe/Serie Methods in Molecular Biology ; v. 212
Zusatzinfo 60 black & white illustrations, 11 colour illustrations, 12 black & white halftones, 48 black & white line drawings
Verlagsort Totowa, NJ
Sprache englisch
Maße 152 x 229 mm
Gewicht 1 g
Einbandart gebunden
Themenwelt Medizin / Pharmazie Studium
Naturwissenschaften Biologie Genetik / Molekularbiologie
ISBN-10 0-89603-968-4 / 0896039684
ISBN-13 978-0-89603-968-1 / 9780896039681
Zustand Neuware
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