Signal Transduction Immunohistochemistry

Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications.- Antibodies That Work Again and Again and Again.- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods.- Acquisition and Post-Processing of Immunohistochemical Images.- Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction.- EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis.- Preservation of Specific Protein Signaling States using Heat Based Stabilizor System.- Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species.- Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages.- Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokinesand Surface Markers.- A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy.- Apoptotic Bodies: Selective Detection in Extracellular Vesicles.- Activation and Differentiation of Mesenchymal Stem Cells.- High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP.- Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry.- Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D.- Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment.- Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors.- Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt.- Automated High Content Screening of g--H2AX Expression in HeLa Cells.