Metagenomics

Construction of Small-Insert and Large-Insert Metagenomic Libraries.- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies.- Construction and Screening Marine Metagenomic Large Insert Libraries.- Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment.- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.- Assessing Bacterial and Fungal Diversity in the Plant’s Endosphere.- Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities.- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production.- Degradation Network Reconstruction Guided by Metagenomic Data.- Novel Tools for the Functional Expression of Metagenomics DNA.- A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries.- Screening for Cellulase Encoding Clones in Metagenomics Libraries.- Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes.- Screening Glycosyltransferases for Polyphenol Modifications.- Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial Communities.- Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity.- Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes.- Screening for N-AHSL-Based-Signaling Interfering Enzymes.- Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites.