Flow Cytometry Basics for the Non-Expert - Christine Goetz, Christopher Hammerbeck, Jody Bonnevier

Flow Cytometry Basics for the Non-Expert

Buch | Hardcover
XVII, 219 Seiten
2018 | 1st ed. 2018
Springer International Publishing (Verlag)
978-3-319-98070-6 (ISBN)
96,29 inkl. MwSt
lt;p>This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly.

The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays.  Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques.

Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data.  This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.  

Christine Goetz, Ph.D., Jody Bonnevier, Ph.D., and Christopher Hammerbeck, Ph.D. R&D Systems, Minneapolis, Minnesota

lt;p>1. Flow Cytometry: Definition, History, and Uses in Biotechnology.- 2. Physics of a Flow Cytometer.- 3. The Language of Flow Cytometry and Experimental Setup.- 4. Fluorochrome Choices for Flow Cytometry.- 5. Compensation.- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry.- 7. Experimental Considerations with Data Sets as Examples.- 8. Cell Enrichment.- 9. Surface and Intracellular Staining Protocols for Flow Cytometry.- 10. Troubleshooting.

Erscheinungsdatum
Reihe/Serie Techniques in Life Science and Biomedicine for the Non-Expert
Zusatzinfo XVII, 219 p. 117 illus., 113 illus. in color.
Verlagsort Cham
Sprache englisch
Maße 155 x 235 mm
Gewicht 526 g
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Laboratoriumsmedizin
Studium Querschnittsbereiche Infektiologie / Immunologie
Schlagworte antibodies • dot plots • flow cytometry • fluorochromes • PMT
ISBN-10 3-319-98070-X / 331998070X
ISBN-13 978-3-319-98070-6 / 9783319980706
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich

von Oliver Schmetzer

Buch | Softcover (2023)
Urban & Fischer in Elsevier (Verlag)
27,00
Antibiotika, Virostatika, Antimykotika, Antiparasitäre Wirkstoffe

von Hans-Reinhard Brodt; Achim Hörauf; Michael Kresken …

Buch (2024)
Thieme (Verlag)
165,00