Influenza Virus
Humana Press Inc. (Verlag)
978-1-4939-8677-4 (ISBN)
Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.
Understanding Influenza.- Clinical Diagnosis of Influenza.- Influenza A Virus Genetic Tools - From Clinical Sample to Molecular Clone.- Propagation and Titration of Influenza Viruses.- Purification and Proteomics of Influenza Virions.- Haploid Screening for the Identification of Host Factors in Virus Infection.- Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies.- Deciphering Virus Entry with Fluorescently-Labeled Viral Particles.- Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape.- Single Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking.- Correlative Light and Electron Microscopy (CLEM) and 3D Methods to Study Architecture of Virus-Infected Cells.- Correlative Light and Electron Microscopy (CLEM) of Influenza Virus Entry and Budding.- Influenza Virus-Liposome Fusion Studies using Fluorescence Dequenching and Cryo-Electron Tomography.- Metal-Tagging Transmission Electron Microscopy and Immunogold Labelling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging.- Live Imaging of Influenza Viral Ribonucleoproteins using Light-Sheet Microscopy.- Purification of Unanchored Polyubiquitin Chains from Influenza Virions.- Measurement of Influenza Virus Polymerase Activity.- In vitro models to study influenza virus and Staphylococcus aureus super-infection on a molecular level.- Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus.- Animal Models in Influenza Research.- Measuring Influenza Virus Infection using Bioluminescent Reporter Viruses for in vivo Imaging and in vitro Replication Assays.- Selection of Antigenically Advanced Variants of Influenza Viruses.- Assessment of Influenza Virus Hemagglutinin Stalk-specific Antibody Responses.- Analyses of Cellular Immune Responses from Ferrets Following Influenza Virus Infection.- Parameter Estimation in Mathematical Models of Viral Infections using R.- Software for Characterizing the Antigenic and Genetic Evolution of Human Influenza Viruses.- Clinical Trials of Influenza Vaccines – Special Challenges.- The Silver Lining in Gain-of-Function Experiments with Pathogens of Pandemic Potential.- Why Do Exceptionally Dangerous Gain-of-Function Experiments in Influenza?.- How Computational Models Enable Mechanistic Insights into Virus Infection.
Erscheinungsdatum | 18.09.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1836 |
Zusatzinfo | 160 Illustrations, color; 23 Illustrations, black and white; XVI, 663 p. 183 illus., 160 illus. in color. With online files/update. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Schlagworte | Microscopy • Vaccine development • Viral proteins • Virology • virus-host interactions |
ISBN-10 | 1-4939-8677-5 / 1493986775 |
ISBN-13 | 978-1-4939-8677-4 / 9781493986774 |
Zustand | Neuware |
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