miRNA Biogenesis

High-throughput characterization of primary microRNA transcripts.- Identifying pri-miRNA transcription start sites.- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics.- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing.- Purification of Microprocessor-associated factors.- Arresting pri-miRNA processing with Target Site Blockers.- MicroRNA analysis using the quantitative real-time PCR reaction.- MicroRNA analysis using next-generation sequencing.- Identification of microRNA precursor-associated proteins.- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput.- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling.- Detection of microRNA-target interactions by Chimera PCR (ChimP).- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA.- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA.- Method for detection of miRNAs in non-model organisms with unreported database.- Detection and Verification of Mammalian Mirtrons by Northern Blotting.- Detecting agotrons in Ago CLIPseq data.