Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors (eBook)

Maximilian Oberleitner was born in 1983 in Bavaria, Germany and graduated from the University of Regensburg with a Diploma in Chemistry in 2008. Based on this doctoral thesis “Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors” he obtained his PhD in Analytical Chemistry in 2016 in the Institute of Analytical Chemistry, Chemo- and Biosensors at the University of Regensburg under the supervision of Prof. Dr. Joachim Wegener. His research work was focused on the development and improvement of substrate-integrated sensors for the analysis of living cells. These sensors enable the monitoring of cell-substrate interactions and the real-time analysis of assays with adherently grown cells in a non-invasive, label-free and – by combinations of the different types of sensors – even in a multi-parametric manner. Since 2015 he works at a global healthcare company in Austria. As project leader in the quality unit he is responsible for stability studies and quality evaluations of antibiotics.

Introduction.- Objectives.- Methods & Instrumentation.- MQCM: Multiple Cytomechanic Sensing.- QCM-ECIS: Combined Viscoelastic and Dielectric Sensing of Cells.- QCM-OCS: Optochemical Sensing of Temperature and pO2 in the Cell Surface Junction.- Summary.- Appendix.