Innate Immune Activation

Emerging concepts in innate immunity.- Bioinformatic Assessment of macrophage activation by the innate immune system.- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system.- Modeling primary human monocytes with the trans-differentiation cell line BLaER1.- Measurement of NF-κB activation in TLR-activated macrophages.- Biochemical isolation of the myddosome from murine macrophages.- Generation of innate immune reporter cell lines using retroviral transduction.- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER).- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis.- Methods for detecting ASC and ASC speck formation.- Measuring innate immune responses to bacterial viability.- Methods to study cell swelling-induced inflammasome activation.- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy.- Quantitative proteomics of secreted proteins.- Simultaneous detection of cellular viability and interleukin-1β secretion from single cells by ELISpot.- Detection and quantification of MAV aggregation via confocal imaging.