PCR Detection of Microbial Pathogens

Real-time Quantitative PCR, Pathogen Detection and MIQE.- Overcoming Inhibition in Real-time Diagnostic PCR.- Quality in the Molecular Microbiology Laboratory.- Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood.- Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods.- Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples.- PCR Detection of Haemophilus influenzae from Respiratory Specimens.-  Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR.- Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens.- Detection of Mycoplasma pneumoniae by Real-time PCR.- Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia.- Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens.- Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples.- Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay.- Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays.- Diagnosis of Clostridium difficile Infection using Real-time PCR.- Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene.- Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification.- PCR Detection of Helicobacter pylori in Clinical Samples.- Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR.- Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli.