Transcriptional Regulation

Genome Wide In Vivo Crosslinking of Sequence Specific Transcription Factors.- Characterization of Complex Regulatory Networks and Identification of Promoter Regulatory Elements in Yeast: “In Silico” and “Wet-Lab” Approaches.- Electrophoretic Mobility Shift Assay Analysis of NFkB Transcriptional Regulation by Nuclear IkBa.- Probing Endogenous RNA Polymerase II Pre-Initiation Complexes by Electrophoretic Mobility Shift Assay.- Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification.- Chromatin Immunoprecipitation (ChIP) Assay as Tool for Analyzing Transcription Factor Activity.- Two-Step Crosslinking for Analysis of Protein-Chromatin Interactions.- Chromatin Immunoprecipitation Analysis of NFkB Transcriptional Regulation by Nuclear IkBa in Human Macrophages.- In Vivo ChIP for the Analysis of Microdissected Tissue Samples.- Quantification of Protein-DNA Interactions by In Vivo Chromatin Immunoprecipitation in Yeast.- Mapping Protein-DNA Interactions Using ChIP-Sequencing.- ChIP and ReChIP Assays:  Investigating Interactions Between Regulatory Proteins, Histone Modifications, and the DNA Sequences to which They Bind.- Transcriptional Regulation of Genes Via Hypoxia-Inducible Factor.- Exchange Protein Directly Activated By Cyclic AMP 1 (Epac1)-Regulated Recruitment of CCAAT/Enhancer Binding Proteins (C/EBPs) to the Suppressor of Cytokine Signalling-3 (SOCS-3) Promoter.- Computational Analysis of Promoter Elements and Chromatin Features in Yeast.- Chromatin Affinity Purification (ChAP).- Determination of Histone Acetylation Status by Chromatin Immunoprecipitation.- Immunostaining of Drosophila Polytene Chromosomes to InvestigateRecruitment of Chromatin Binding Proteins.- Detection of Transcriptional Activators, Co-Activators, and Chromatin Remodeling by Chromatin Immunoprecipitation Coupled with Real-Time PCR.- Chromatin Endogenous Cleavage and Psoralen Crosslinking Assays to Analyze rRNA Gene Chromatin In Vivo.- UV-Induced DNA Damage and DNA Repair in Ribosomal Genes Chromatin.- Analysis of SUC2 Promoter Structure by Nucleosome Scanning.- Chromatin Immunoprecipitation of Mouse Embryos.- Chromatin Immunoprecipitation (ChIP) in Mouse Hippocampal Cells and Tissues.- Approaches for Studying Nucleosome Movement by ATP-Dependent Chromatin Remodeling Complexes.- Mapping Protein-DNA and Protein-Protein Interactions of ATP-Dependent Chromatin Remodelers.- Evaluation of Histone Modifying Enzymes in Stem Cell Populations.- Purification of Multiprotein Histone Acetyltransferase Complexes.- Reconstitution of Active and Stoichiometric Multisubunit Lysine Acetyltransferase Complexes in Insect Cells.- Affinity Purification of MLL3/MLL4 Histone H3K4 Methyltransferase Complex.- Methods for Analyzing Histone Citrullination in Chromatin Structure and Gene Regulation.- Analysis of mRNA Abundance and Stability by Ribonuclease Protection Assay.- Array-Based Nuclear Run-On (ANRO) Analysis.- In Vivo Run on Assays to Monitor Nascent Precursor RNA Transcripts.- Genome Wide Full-Length Transcript Analysis Using 5’ and 3’ Paired-End-Tag Next Generation Sequencing (RNA-PET).- Analysis of Cotranscriptionnal RNA Processing by RNA-ChIP Assay.- Quantitative Analysis of Transcription Elongation by RNA Polymerase I In Vitro.- Detection and Characterization of Transcription Termination.- Promoter-Associated Noncoding RNA from the CCND1Promoter.