Immunoelectron Microscopy

Molecular Toolbox.- Protein Antigen Expression in Escherichia coli for Antibody Production.- Expression of Epitope-Tagged Proteins in Plants.- Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts.- Transient Expression of Epitope-Tagged Proteins in Mammalian Cells.- Production and Purification of Polyclonal Antibodies.- Production and Purification of Monoclonal Antibodies.- Production of Antipeptide Antibodies.- Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab’)2, and Streptavidin.- Microscopy Toolbox.- Immunoelectron Microscopy of Chemically Fixed Developing Plant Embryos.- Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slices.- Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells.- Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.- In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy.- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells.- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae.- High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling.- Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tissue Using Horseradish Peroxidase-Conjugated Lectin.- Pre-embedding Nanogold Silver and Gold Intensification.- The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedure Based on Heat-Induced Antigen Retrieval.- Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells.- Serial Section Immunoelectron Microscopy of Algal Cells.- Freeze-Etch Electron Tomography for the PlasmaMembrane Interface.- Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy.- Immunogold Labelling for Scanning Electron Microscopy.- Horseradish Peroxidase as a Reporter Gene and as a Cell-Organelle-Specific Marker in Correlative Light-Electron Microscopy.- Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescently Labeled with FM1-43.