Targeted Biomarker Quantitation by LC-MS (eBook)

Naidong Weng, PhD, is Scientific Director, Janssen Fellow, and Head of Bioanalytical Chemistry and Pharmacokinetics within Department of Pharmacokinetics, Dynamics and Metabolism at US East Coast, Janssen Research & Development, Johnson and Johnson. He has over 25 years of experiences on quantitative bioanalysis. His research interest includes using HILIC-MS/MS for quantitation of highly polar analytes as well as chiral analysis. He has published more than 110 journal papers and book chapters. Wenying Jian, PhD, a Senior Principal Scientist of Bioanalytical Chemistry and Pharmacokinetics within Department of Pharmacokinetics, Dynamics and Metabolism at US East Coast, Janssen Research & Development, Johnson and Johnson. Her research experience and interest center on application of advanced LC-MS methodologies in detection, identification, and quantitation of endogenous molecules, drugs and their metabolites, including small and large molecules, and in complicated biological matrices. She has published more than 40 journal papers and book chapters.

List of Contributors

Preface

Abbreviations

Chapter Authors

Part 1. Overview

1. Overview of Targeted Quantitation of Biomarkers and Its Application

2. Translational Application of Biomarkers

3. Current Regulatory Guidance Pertaining Biomarker Assay Establishment and Industrial Practice of Fit-for-Purpose and Tiered Approach

4. Modern Liquid Chromatography and Mass Spectrometry for Targeted Biomarker Quantitation

5. Comparison between LC-MS and Ligand Binding Assay Approaches for Biomarker Quantification

6. Sample Preparation Methods for Targeted Biomarker Quantification by LC-MS

7. Overcome the Endogenous Levels in Biomarker Quantitation Using LC-MS

Part 2. Challenges and Approaches

8. Sample Collection for Targeted Biomarker Quantitation by LC-MS

9. Nonspecific Binding in LC-MS Bioanalysis

10. Strategies for Improving Sensitivity for Targeted Quantitation by LC-MS

11. Strategies to Improve Specificity for Targeted Biomarker Quantitation by LC-MS

12. Biomarker Quantitation Using Relative Approaches

13. Targeted Quantification of Amino Acid Biomarkers Using LC-MS

Part 3. Applications

14. Targeted Quantification of Peptide Biomarkers - A Case Study of Amyloid Peptides

15. Targeted Protein Biomarker Quantitation by LC-MS

16. Glycoprotein Biomarkers

17. Targeted Lipid Biomarker Quantitation Using Liquid Chromatography - Mass Spectrometry (LC-MS)

18. Targeted LC-MS Quantification of Androgens and Estrogens for Biomarker Development

19. Steroid Biomarkers

20. Bile Acids as Biomarkers

21. Biomarkers for Vitamin Status and Deficiency - LC-MS Based Approach

22. Quantitation of acyl-coenzyme A thioesters as metabolic biomarkers

23. Neurotransmitter Biomarkers

24. Targeted Quantification of Carbohydrate Biomarkers Using LC-MS

25. Nucleoside/Nucleotide Biomarkers

26. LC-MS of RNA Biomarkers