Urothelial Carcinoma -

Urothelial Carcinoma

Methods and Protocols
Buch | Hardcover
362 Seiten
2017 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-7233-3 (ISBN)
171,19 inkl. MwSt
This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis.  Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.

Analysis of Chromosomal Alterations in Urothelial Carcinoma.- Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma.- A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer.- Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers.- Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel.- Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models.- Algorithm for the Automated Evaluation of NAT2 Genotypes.- Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma.- Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species.- Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods.- In Vitro Differentiation and Propagation of Urothelium from Pluripotent Stem Cell Lines.- Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method.- The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model.- Patient-derived Bladder Cancer Xenografts.- Orthotopic Mouse Models of Urothelial Cancer.- Quantification of microRNAs in Urine-derived Specimens.- Quantitative RNA Analysis from Urine using Real Time PCR.- DNA Methylation Analysis from Body Fluids.- Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma.- Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin.- Epigenetic Treatment Options in Urothelial Carcinoma.- Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum.- Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer.- Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1655
Zusatzinfo 49 Illustrations, color; 24 Illustrations, black and white; XIV, 362 p. 73 illus., 49 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Onkologie
Medizin / Pharmazie Studium
Schlagworte bladder • Carcinomas • immunotherapy • next generation sequencing techniques • Urinary
ISBN-10 1-4939-7233-2 / 1493972332
ISBN-13 978-1-4939-7233-3 / 9781493972333
Zustand Neuware
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