DNA Cloning 1: A Practical Approach -

DNA Cloning 1: A Practical Approach

Core Techniques

D. M. Glover, B. D. Hames (Herausgeber)

Buch | Softcover
288 Seiten
1995 | 2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963476-7 (ISBN)
89,95 inkl. MwSt
This book is one of four which together are considerably updated and totally revised texts that arose out of the very successful "DNA Cloning, Volume I-IV" books in the Practical Approach series. This particular book describes all the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers.

These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

1. Techniques for transformation of E.coli ; 2. Lambda replacement vectors in the construction of genomic DNA libraries ; 3. Procedures for cDNA cloning ; 4. Making probes ; 5. Using cloned DNAs to analyse gene organization and expression ; 6. Choices of single-stranded vectors and their application for in vitro mutagenesis ; 7. PCR and its applications ; 8. DNA sequencing

Erscheint lt. Verlag 11.5.1995
Reihe/Serie Practical Approach Series ; 148
Zusatzinfo halftones, line figures, tables
Verlagsort Oxford
Sprache englisch
Maße 157 x 235 mm
Gewicht 499 g
Themenwelt Medizin / Pharmazie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
Technik Umwelttechnik / Biotechnologie
ISBN-10 0-19-963476-9 / 0199634769
ISBN-13 978-0-19-963476-7 / 9780199634767
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich