Decoding the Antibody Repertoire

Brandon DeKosky is an assistant professor at the Departments of Pharmaceutical Chemistry, Chemical & Petroleum Engineering, and the Kansas Vaccine Institute at the University of Kansas.  Dr. DeKosky attained his Ph.D. in the lab of George Georgiou at the University of Texas at Austin where he developed new technologies for sequencing complete antibody variable regions from single B cells.  Dr. DeKosky performed postdoctoral studies in the lab of John Mascola at the NIAID Vaccine Research Center, applying high-throughput technologies to accelerate public health vaccine development.  His lab at KU seeks to leverage high-throughput platforms to understand the features of adaptive immune protection and develop novel strategies to combat human diseases.

Background.- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire.- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire.- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors.- Conclusions and Future Perspectives.- Appendices.