Zygotic Genome Activation

Clearance of Maternal RNAs: Not a Mummy’s Embryo Anymore.- Link of Zygotic Genome Activation and Cell Cycle Control.- Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis.- Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR.- Detection of miRNA in Mammalian Oocytes and Embryos.- Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method.- Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation.- Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos.- RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos.- Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry.- Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo.- Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos.- Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation.- Targeted Gene Knockdown in Early Embryos using siRNA.- Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput.- CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine.- Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development.- Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.