Flow Cytometry in Neoplastic Hematology

Wojciech Gorczyca MD, PhD, currently works at Bioreference Laboratories as Medical Director of Flow Cytometry and Chief for Hematopathology Services. A noted authority in clinical flow cytometry and hematopathology, he served for more than ten years as a hematopathologist and director of hematopathology with Genzyme Genetics, Inc. / IMPATH, Inc. and later with CSI Laboratories’ medical team where he worked as director of flow cytometry.

Preface to the third edition. Abbreviations. Introduction. Gating strategies. Classification of hematopoietic neoplasms. Identification of clonal B-cell population. Identification of atypical T-cell population. Identification of myeloblasts. Identification of B-lymphoblasts. Identification of T-lymphoblasts. Antigen expression during myeloid and lymphoid maturation. Identification of myelodysplastic syndrome by flow cytometry. Phenotypic markers. Morphologic-flow cytometric correlation. Molecular-flow cytometric correlation. Flow cytometry limitations. Phenotypic classification of B-cell lymphoproliferations. Mature B-cell lymphoproliferations. Plasma cell neoplasms. Mature T/NK-cell lymphoproliferations. Hodgkin lymphoma. B-lymphoblastic leukemia (B-ALL). T-lymphoblastic leukemia (T-ALL). Myelodysplastic syndromes. Chronic myelomonocytic leukemia and other myelodysplastic/myeloproliferative neoplasms. Myeloproliferative neoplasms. Acute myeloid leukemia: introduction. Acute myeloid leukemia: recurrent genetic changes. Acute myeloid leukemia: not otherwise specified. Other neoplasms.