The Isolated hepatocyte

Preface
1 Regulation of Liver Growth: Historical Perspectives and Future Directions
I. Introduction
II. Whole-Animal Studies
III. Hepatocyte Cultures
IV. Future Directions
References
2 Regulation of Sulfation and Glucuronidation of Xenobiotics in Periportal and Pericentral Regions of the Liver Lobule
I. Introduction
II. Sulfation and Glucuronidation in Periportal and Pericentral Regions of the Liver Lobule
III. Conclusion
References
3 Toxicology Studies in Cultured Hepatocytes from Various Species
I. Introduction
II. Hepatocyte Primary Cultures: Methods
III. Uses of Hepatocyte Primary Cultures for Xenobiotic Studies
IV. Conclusions
References
4 Cytochrome P-450-Dependent Monooxygenase Systems in Mouse Hepatocytes
I. Introduction
II. Methods Used to Study Drug Biotransformation in Isolated Hepatic Cells
III. Applications
IV. Conclusions
References
5 Control of Hepatocyte Proliferation in Vitro
I. Introduction
II. Hepatocyte Isolation and Culture
III. Epidermal Growth Factor
IV. Norepinephrine
V. Factors Derived from Serum or Platelets
VI. Factors Derived from Normal Liver Cells
VII. Factors Derived from Neoplastic Liver Cells
References
6 Cytotoxicity Measures: Choices and Methods
I. Introduction
II. Plasma Membrane Integrity
III. Subcellular Effects
IV. Data Presentation
V. In Vivo/in Vitro Correspondence
VI. Methods
References
7 Metals, Hepatocytes, and Toxicology
I. Introduction
II. Uptake and Efflux of Metals
III. Use of Hepatocytes to Study Chelators
IV. Toxicity of Metals
V. Interactions among Metals
VI. Mechanisms of Toxicity
VII. Conclusion
References
8 The Metabolism and Toxicity of Xenobiotics in a Primary Culture System of Postnatal Rat Hepatocytes
I. Introduction
II. Retention and Maintenance of Liver-Specific Differentiated Functions in Primary Culture of Postnatal Rat Hepatocytes
III. Toxicity Evaluation of Xenobiotics in Cultured Hepatocytes
IV. Metabolic Activation and Toxicity of Xenobiotics in Cultured Hepatocytes
V. Conclusions
References
9 The Analysis of Carcinogen-Induced Pleiotropic Drug Resistance in Rat Hepatocytes
I. Introduction
II. Study of Drug Resistance in Carcinogen-Altered Hepatocytes
III. Cytotoxicity Studies in Vitro
IV. Cellular Pharmacokinetics of Adriamycin in Sensitive and Resistant Hepatocytes
V. Membrane Actions of Adriamycin
VI. Conclusions
References
10 Measurement of Chemically Induced DNA Repair in Hepatocytes in Vivo and in Vitro as an Indicator of Carcinogenic Potential
I. Introduction
II. DNA Repair
III. Procedures for in Vitro and in Vivo Rat Hepatocyte DNA Repair Assays
References
11 Genotoxicity Studies with Human Hepatocytes
I. Introduction
II. Isolation and Culture of Human Hepatocytes
III. Hepatocyte Cell Culture
IV. Cryopreservation of Human Hepatocytes
V. Genotoxicity Studies with Human Hepatocytes
VI. Xenobiotic Metabolism by Human Hepatocytes
VII. Conclusions
References
Index