Cytokines
Oxford University Press (Verlag)
978-0-19-963566-5 (ISBN)
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Cytokines are proteins that control communication between cells and they are important in immunity, inflamation, tissue development, tissue repair, and cancer. This book is the new, revised edition of "Cytokines: A Practical Approach" published in 1991. It provides detailed, up-to-date practical information for the study of cytokines and cytokine-like activity and is also a guide to identifying and characterizing individual cytokines and provides a review of
cytokine biology. In addition, in providing a guide to some of the most useful techniques in cell and molecular biology, immunology, and biochemistry, it will be useful to workers in many different disciplines of biomedical research.
In this second edition, many important new techniques have been added including methods for developing cytokine transgenic mice, measuring soluble cytokine receptors, and assays for newly discovered cytokines (up to 1L-14). Each chapter has been updated in line with current experiments.
1. Cloning and expression of cytokine genes ; 2. Southern blotting of cytokine genes ; 3. Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA ; 4. Polymerase chain reaction to quantitate cytokine mRNA ; 5. Comparative polymerase chain reaction ; 6. Receptor binding studies ; 7. Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases ; 8. Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells ; 9. Antiviral activity of cytokines ; 10. Assays for human B cell responses to cytokines ; 11. T cells and cytokines ; 12. The generation and quantitation of cell-mediated toxicity ; 13. Assays for chemotaxis ; 14. Cytokine regulation of endothelial cells ; 15. Assays for haematopoietic growth factors ; 16. Assays for macrophage activation by cytokines ; 17. Measurement of proliferative, cytolytic and cytostatic activity of cytokines ; 18. Production of cytokine transgenic and knockout mice ; 19. Development of antibodies to cytokines ; 20. Visualizing the production of cytokines and their receptors by single human cells ; 21. Quantitative biological assays for individual cytokines ; 22. RIA, IRMA and ELISA assays for cytokines and their soluble receptors
| Erscheint lt. Verlag | 28.9.1995 |
|---|---|
| Reihe/Serie | Practical Approach Series ; 155 |
| Zusatzinfo | halftones, line figures, tables |
| Verlagsort | Oxford |
| Sprache | englisch |
| Maße | 156 x 235 mm |
| Gewicht | 674 g |
| Themenwelt | Studium ► 1. Studienabschnitt (Vorklinik) ► Histologie / Embryologie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-19-963566-8 / 0199635668 |
| ISBN-13 | 978-0-19-963566-5 / 9780199635665 |
| Zustand | Neuware |
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