Therapeutic Applications of Ribozymes and Riboswitches

Identification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites.- Screening Inhibitory potential of anti-HIV RT RNA Aptamers.- Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA.- Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells.- Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target.- Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells.- Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment.- Characterization of Hairpin Ribozyme Reactions.- Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal.- Structure-based Virtual Screening for the Identification of RNA-binding Ligands.- Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays.- Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection.- Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs.- Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells.- Screening Assays to Identify Artificial glmS Ribozyme Activators.- Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS).- Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies.- Cell Internalization SELEX:  In Vitro Selection for Molecules that Internalize into Cells.- DNA Electronic Switches Based on Analyte-Responsive Aptamers.