Homing Endonucleases

Homing Endonucleases:  From Genetic Anomalies to Programmable Genomic Clippers.- Bioinformatic Identification of Homing Endonucleases and Their Target Sites.- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein.- Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE.- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis.- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases.- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection.- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile.- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling.- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries.- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display.- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes.- Bioinformatics Identification of Coevolving Residues.- Identification and Analysis of Genomic Homing Endonuclease Target Sites.- Redesigning the Specificity of Protein-DNA Interactions with Rosetta.