Bacterial Artificial Chromosomes
Humana Press Inc. (Verlag)
978-1-4939-5430-8 (ISBN)
Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.
From Selective Full-Length Genes Isolation By TAR Cloning In Yeast To Their Expression From HAC Vectors In Human Cells.- Recombineering Linear BACs.- BAC Sequencing Using Pooled Methods.- Making BAC Transgene Constructs With Lambda-Red Recombineering System For Transgenic Animals Or Cell Lines.- Directing Enhancer-Traps And Itol2 End Sequences To Deleted BAC Ends With Loxp- and Lox511-Tn10 Transposons.- Recombining Overlapping Bacs Into Single Large Bacs.- Generation Of Knockout Alleles By RFLP Based BAC Targeting Of Polymorphic Embryonic Stem Cells.- Herpesvirus Mutagenesis Facilitated By Infectious Bacterial Artificial Chromosomes (Ibacs).- Conversion Of BAC Clones Into Binary BAC (BIBAC) Vectors and Their Delivery Into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens.- Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomes.- A Recombineering-Based Gene Tagging System For Arabidopsis.- BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies.- Bacs-On-BeadsTM (BobsTM) Assay for the Genetic Evaluation of Prenatal Samples And Products of Conception.- New BAC Probe Set To Narrow Down Chromosomal Breakpoints In Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions.- BAC-Probes Applied For Characterization Of Fragile Sites (FS).- Application Of BAC-Probes To Visualize Copy Number Variants (CNVs).- Site-Specific Integration Of Bacterial Artificial Chromosomes Into Human Cells.- Generation Of BAC Reporter Cell Lines For Drug Discovery.
Erscheinungsdatum | 14.08.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 1227 |
Zusatzinfo | 36 Illustrations, color; 12 Illustrations, black and white; XI, 348 p. 48 illus., 36 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | achieving gene expression • BAC construction • BACs • BACs in model organisms • drug discovery • medical genetics |
ISBN-10 | 1-4939-5430-X / 149395430X |
ISBN-13 | 978-1-4939-5430-8 / 9781493954308 |
Zustand | Neuware |
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