Plant Virology Protocols

Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method.- RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers.- New Method to Isolate Total dsRNA.- Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses.- Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP).- Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector.- Detection and Analysis of Non-Retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes.- Detection of Plant Viruses in Natural Environments by Using RNA-Seq.- Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA.- Drawing siRNAs of Viral Origin out from Plant siRNAs Libraries.- Viral Detection by High-Throughput Sequencing.- Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing.- Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing.- SuperSAGE as an Analytical Tool for Host and Viral Gene Expression.- Microarray Analysis of R-Gene-Mediated Resistance to Viruses.- Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus.- Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors.- Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones.- Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control.- Detection of Plant Virus in Meristem by Immunohistochemistry and In Situ Hybridization.