Recombinant Protein Production in Yeast

Optimising Yeast as a Host for Recombinant Protein Production( review).-Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review).-Preparation of Pichia pastoris Expression Plasmids.-Preparation of Saccharomyces cerevisiae Expression Plasmids.-Codon Optimisation for Heterologous Gene Expression in Yeast.-Yeast Transformation to Generate High-yielding Clones.-Screening for High-yielding Pichia pastoris Clones: the Production of G Protein-Coupled Receptors as a Case Study.-Screening for High-yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins.-The Effect of Antifoam Addition on Protein Production Yields.-Setting up a Bioreactor for Recombinant Protein Production in Yeast.-The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (review).-On-line Analysis and Process Control in Recombinant Protein Production (review).-Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Off-line Estimations of Dry Cell Mass and Cell Counts.-On-line Monitoring of Biomass Accumulation in Recombinant Yeast Cultures.-Optimising P. pastoris Induction-Optimizing S. cerevisiae Induction Regimes.-Large Scale Production of Membrane Proteins in Pichia pastoris: The Production of G Protein-coupled Receptors as a Case Study.-Large-scale Production of Membrane Proteins in Saccharomyces cerevisiae: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteinsl.-Large Scale Production of Secreted Proteins in Pichia pastoris.-Disruption of Yeast Cells to Isolate Recombinant Proteins.