Nanoparticles in Biology and Medicine
Humana Press Inc. (Verlag)
978-1-4939-6247-1 (ISBN)
Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.
Procedures for the Synthesis and Capping of Metal Nanoparticles.- Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications.- Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis.- Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays.- Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues.- RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics.- Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins.- Synthesis of Semiconductor Nanoparticles.- Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature.- Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods.- In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing.- Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis.- The Use of Quantum Dots for Immunochemistry Applications.- Quantum Dots for Labelling Live Cells.- Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots.- The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry.- Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging.- Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging.- Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry.- Synthesis, Application, and Tracking ofMagnetic Carbon-Coated Nanoparticles in Plants.- Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”).- Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery.- Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery.- Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases.- Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency.- Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications.- Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection.- Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications.- Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins.- Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles.- Extraction of Organic Nanoparticles from Plants.- Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay.- Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression.- Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells.- Assessment of Toxicity of Nanoparticles Using Insects as Biological Models.- Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues.- Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles.- Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction.- Microscopy Imaging Methods for theDetection of Silver and Titanium Nanoparticles Within Cells.- Detection of TiO2 Nanoparticles in Cells by Flow Cytometry.- NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions.- Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis.- Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules.
Erscheinungsdatum | 27.06.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 906 |
Zusatzinfo | XVII, 555 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie |
Medizin / Pharmazie ► Pharmazie | |
ISBN-10 | 1-4939-6247-7 / 1493962477 |
ISBN-13 | 978-1-4939-6247-1 / 9781493962471 |
Zustand | Neuware |
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