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Colloidal Gold

Principles, Method and Application

M. A. Hayat (Herausgeber)

Buch | Hardcover
421 Seiten
1990
Academic Press Inc (Verlag)
978-0-12-333929-4 (ISBN)
129,95 inkl. MwSt
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This volume covers the principles and methodology of colloidal gold in microscopy. It explains how this method can be applied to most microscopial systems, including optical microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy.
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field.

Some basic concepts for the choice methods, E.Kellenberger and M.A.Hayat; techniques for the production of monoclonal and polyclonal antibodies, K.J.Morrow, et al; lowicryl resins, W.Villiger; polar and apolar lowicryl resin for immunolabelling, M.Durrenberger et al; comparable yield of immunolabel by resin sections and thawed cryosections, Y.-D.Stierhof et al; colloidal gold labeling for determining cell surface area, V.Herzog and T.Kehle; quantitation of colloidal gold by electron microscopy, T.Kehle and V.Herzog; simultaneous demonstration of antigens on outer and protoplasmic surfaces of the plasma membrane by replica immunocytochemistry, G.Rutter and H.Hohenberg; double labelling of antigenic sites on cell surfaces imaged with backscattered electrons, E.Namork; location and identification of colloidal gold particles with an energy dispersive analyzer, S.Eskelinen and R.Peura; use of dinitrophenol IgG conjugates - immunogold labelling of cellular antigens on thin sections of osmicated and epon-embedded specimens, R.Pathak and R.G.W.Anderson; preparation and application of insulin-gold complex, R.M.Smith and L.Jarett; micro-injection of colloidal gold, S.I.Dworetzky; combined diaminobenzidine-colloidal gold staining, H.C.F.Mutasa; colloidal gold techniques and the immunocytochemistry of antigen-antibody reactions at interfaces, H.Nygren and M.Werthen; colloidal gold labelling for diagnostic pathology, G.A.Herrara and R.L.Lott; immunogold silver staining using microwave irradiation, M.E.Boon et al; a correlative approach to colloidal gold labelling with video-enhanced light microscopy, low voltage scanning electron microscopy, and high voltage electron microscopy, S.L.Goodman et al.

Reihe/Serie Colloidal Gold: Principles, Methods & Application
Zusatzinfo Illustrations
Verlagsort San Diego
Sprache englisch
Maße 156 x 235 mm
Gewicht 908 g
Themenwelt Studium 1. Studienabschnitt (Vorklinik) Histologie / Embryologie
Naturwissenschaften Biologie Zellbiologie
Naturwissenschaften Chemie Organische Chemie
ISBN-10 0-12-333929-4 / 0123339294
ISBN-13 978-0-12-333929-4 / 9780123339294
Zustand Neuware
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