Clinical Applications of Leukocyte Depletion

Part 1:Production aspects: Collection of leukocyte-depleted platelets using the CS-3000 Plus separator and TNX-6 chamber; Comparison of a "top and bottom" system with a conventional quadruple-bag system for blood-component preparation and storage; Preparation of leukocyte-poor blood components by the nonbutton platelet concnetrate method using quadruple- and quintuple-bag systems; Properties of the Factor VIII/von Willebreand factor complex in stor ed banked blood: Part 2: Laboratory Aspects: Multicenter studies on leukocyte depletion by filtration of red cell and platelet concentrates: methodology and controls; Quality control of leukocyte depletion in blood banking with a flow cytometer: flow cytometry to determine low concentrations of white cells in leukocyte-poor platelet concentrates; Comparison of highly sensitive methods used to count residual leukocytes in filtered red cell and platelet concentrates; Flow cytometric analysis of residual leukocytes in filtered blood components: Part 3: Basic Aspects: Mechanism of leukocyte removal with fibers; Mechanism of leukocyte removal by porous material; Develo pment of polymeric adsorbents for separation of lymphocyte subpopulations; Leukocyte depletion of HTLV-1 carrier red cell concentrates by filters; Mechanisms if immune responses to allo-MHC Class I; UV-B irradiation to platelet concentrates and its effects in vio and in vitro: Part 4: Clinical Aspects: The effects of leukocyte depletion on alloimmunization by platelet transfusions; Leukocyte depletion of red cell and platelet concentrates: clinical importance to patients awaiting organ transplantation; Use of leukocyte-depleting filters for prevention of HLA alloimmunization and refractoriness to platelet trnsfusion; Prevention of immunologic and infectious complications of transfusion by leukocyte depletion; Role of leukocyte depletion from platelet concentrates in reducing HLA alloimmunization and platelet refractoriness in polytransfused patients: a prospective multicenter randomized study in Japan; Leukocyte depletion of blood and blood components: current problems and solutions.