Cajal and de Castro's Neurohistological Methods

Miguel A. Merhcan is a Professor of Histology at the University of Salamanca in Spain. Javier De Felipe is Profesor de Investigación at the Instituto Cajal (Consejo Superior de Investigaciones Científicas) and Centro de Tecnología Biomédica in Madrid, Spain. Fernando de Castro is a member of the Instituto Cajal, Grupo de Neurobiologia del Desarrollo-GNDe and Instituto Cajal (Consejo Superior de Investigaciones Científicas) in Madrid, Spain.

Principles of Micrographical Techniques for the Nervous System
Dedication
Preliminary Information
List of Plates

Section I
Historical Context
CHAPTER I
Authors and Their Backgrounds by JAIME A. MERCHAN and JORGE PRIETO
Section II
Basic Histological Techniques

Chapter II
The Microscope and the Fixatives
I. Microscope
II. Fixatives
Formaldehyde-based Fixatives
Fixatives with Osmic Acid
Fixatives Based on Hypnotic Substances
CHAPTER III
Decalcifying Agents
CHAPTER IV
General Dissociation Procedures
Dissociation
Sectioning
Frozen Section Procedure (EN 8)
Celloidin and Collodion Embedding
Paraffin Embedding
Gelatin Embedding
Superficial Paraffin Imbedding
CHAPTER V
Handling and Mounting Serial Sections
Sections of celloidin-embedded material
Sections of paraffin-embedded material
CHAPTER VI
General Staining Methods
Some Remarks on the Staining Process
Sources and Composition of Dyes
CHAPTER VII
Formulae for Carmine and Haematoxylin
Double and Triple Stains
Modifications of the Iron-Haematoxylin. Methods for the Staining of Mitochondria
CHAPTER VIII
Section Mounting and Preservation
Mounting Sections that Require Previous Dehydration and Clarifying
Section Mounting without Dehydration and Clarifying
Section III
Special Techniques
CHAPTER IX
Methods for the Demonstration of Neuronal Morphology
CHAPTER X
Continuation of the Methods for Demonstrating the Morphology of Neurons
Fixation, Sectioning and Mounting Techniques for Methylene Blue Stained Material
CHAPTER XI
Methods to Study the Structure of the Nerve Cell
Nissl Chromatic Bodies
Methods for Staining the Neurofibrils
Silver Impregnation Methods
Impregnation Of The Golgi Apparatus
Demonstration of Plastosomes, Centrosome, Oxidases and Pigmentary Spherules
Nucleus and Cell Membrane
CHAPTER XII
Staining of Neuronal Axons in the Centres
CHAPTER XIII
Myelin Staining
CHAPTER XIV
Colouration of the Macroglia, Microglia and Oligodendroglia
Macroglia
Demonstration of the Gliosomes and Mitochondria
Impregnation of Oligodendroglia and Microglia
CHAPTERXV
Methods to Demonstrate the Connective Tissue
Methods For Staining The Connective Tissue
Impregnation Methods for Connective Tissue
Supravital Staining with Acidic Dyes
CHAPTER XVI
Methods for the Demonstration of Substances Produced by Alterations of the Cell Metabolism
Demonstration of some Cell Granules
Demonstration of Fats and Lipoid Substances
Demonstration of Ferric Pigments
Demonstration of non-Ferric Pigments
Glycogen Demonstration
Demonstration of Amyloid Substance and Corpora Amylacea
Demonstration of Hyaline Degeneration
Calcareous Substances
CHAPTER XVII
Methods for the Demonstration of the Peripheral Nerves under both Normal and Pathological Conditions
Demonstration of Ranvier Nodes
Methods to Stain the Schwann Cells
Methods for Myelin Staining
Lantermann Incisures; Spiral Apparatus; Spinous Double Bracelet; Neurokeratin Network (EN 48)
Demonstration of the Cylinder-Axis and Neurofibrils
Demonstration of the Connective Tissue of Nerve Trunks (Endoneurium, Perineurium and Epineurium)
CHAPTER XVIII
Methods for the Demonstration of Peripheral Nerve Endings
Gold Chloride Methods
Demonstration of Nerve Endings with the Golgi Method
Demonstration of Peripheral Nerve Endings with Ehrlich's Methylene Blue Procedure
Neurofibrillary Methods Capable of Demonstrating the Nerve Endings
Methods to Demonstrate the Nerve Trunks and Nerve Endings in Organs Protected by Bone Tissue
Procedures for Staining the Sympathetic and Dorsal Root Ganglia
CHAPTERXIX
Techniques for the Demonstration of the Nervous Tissue of Invertebrates
Methods Capable of Revealing the Morphology of Neurons
Methods for Staining Neurofibrils
CHAPTER XX
Methods for Demonstrating some Pathogenic Microorganisms
Demonstration of the Syphilis Treponema
Demonstration of the Trypanosomes in Sleeping Sickness
Colouration of Negri Bodies in Rabies
Demonstration of the Tuberculosis Bacillus

Editors' Notes
Vocabulary by the Editors
Bibliography