Microbiology

James G. Cappuccino is a retired professor emeritus of microbiology from the Department of Biology of the State University of New York at Rockland Community College in Suffern, New York. He received his B.S degree from Seton Hall University in 1951, his M.S degree (1955) and his Ph.D. (1957) in microbiology from Rutgers University in New Brunswick, New Jersey. He was the author and co-author of numerous papers in the area of cancer research, and was a member of the faculty of the Sloan Kettering Division of the Graduate school of Medical Sciences at Cornell University where he taught microbiology from 1957-1970. From there, he taught microbiology, parasitology and clinical chemistry at SUNY Rockland until 2008. He was awarded the status of emeritus professor in 2012. In 1991 he was the recipient of the Chancellor's award from the State University of New York for Excellence in Teaching. He is an emeritus member of the American Society for Cancer Research (ASCR) and an emeritus member of American society for Microbiology (ASM). When not writing he enjoys spending time with his wife Elaine and their family at their summer home at the New Jersey shore. He also enjoys theater, literature, and the quiet hour in his wood working shop. Chad T. Welsh holds a Ph.D. in Microbiology and Immunology from the University of Louisville, School of Medicine, also an M.S. and B.S. in Biology from Middle Tennessee State University. Currently he is the Chair of the Division of Biological and Earth Sciences at Lindenwood University in St. Charles, MO where he has the privilege of teaching Microbiology, both for non-majors and majors, Cellular Immunology, Parasitology, and many other courses since 2010. His research interests fall within bacteriology, eukaryotic cell biology, and immunology, focusing primarily on intracellular eukaryotic signals in response to pulmonary bacterial pathogens. His mentored research projects with his students have spanned the interest areas of soil microbial ecology, immune stress responses in collegiate athletes, oral bacterial flora communities, and many others.

Laboratory Safety

Laboratory Protocol



Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms

1. Culture Transfer Techniques

2. Techniques for Isolation of Pure Cultures

3. Cultural Characteristics of Microorganisms



Part 2 Microscopy

4. Microscopic Examination of Stained Cell Preparations

5. Microscopic Exami na tion of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount



Part 3 Bacterial Staining

6. Preparation of Bacterial Smears

7. Simple Staining

8. Negative Staining

9. Gram Stain

10. Acid-Fast Stain

11. Differential Staining for Visualization of Bacterial Cell Structures



Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

12. Nutritional Requirements: Media for the Routine Cultivation of Bacteria

13. Use of Differential, Selective, and Enriched Media

14. Physical Factors: Temperature

15. Physical Factors: pH of the Extracellular Environment

16. Physical Factors: Atmospheric Oxygen Requirements

17. Techniques for the Cultivation of Anaerobic Microorganisms

18. Serial Dilution-Agar Plate Procedure to Quantitate Viable Cells

19. The Bacterial Growth Curve



Part 5 Biochemical Activities of Microorganisms

20. Extracellular Enzymatic Activities of Microorganisms

21. Carbohydrate Fermentation

22. Triple Sugar-Iron Agar Test

23. IMViC Test

24. Hydrogen Sulfide Test

25. Urease Test

26. Litmus-Milk Reactions

27. Nitrate Reduction Test

28. Catalase Test

29. Oxidase Test

30. Utilization of Amino Acids

31. Genus Identification of Unknown Bacterial Cultures



Part 6 The Protozoa

32. Free-Living Protozoa

33. Parasitic Protozoa



Part 7 The Fungi

34. Cultivation and Morphology of Molds

35. Yeast Morphology, Cultural Characteristics, and Reproduction

36. Identification of Unknown Fungi



Part 8 The Viruses

37. Cultivation and Enumeration of Bacteriophages

38. Isolation of Coliphages from Raw Sewage