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PTEN

Methods and Protocols

Leonardo Salmena, Vuk Stambolic (Herausgeber)

Buch | Hardcover

324 Seiten

2015 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3297-9 (ISBN)

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This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.

Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9

PTEN: History of a Tumor Suppressor.- PTEN at 18: Still Growing.- Immunopathologic Assessment of PTEN Expression.- Measurement of PTEN by Flow Cytometry.- Detecting PTEN and PI3K Signalling in Brain.- Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome.- Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.- Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET.- Methods for the Identification of PTEN-Targeting MicroRNAs.- Post-Transcriptional Regulation of PTEN by Competing Endogenous RNAs.- In Cell and In Vitro Assays to Measure PTEN Ubiquitination.- Assessing PTEN Subcellular Localization.- Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum.- Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry.- Methods for PTEN in Stem Cells and Cancer Stem Cells.- Modeling Cancer Associated Mutations of PTEN in Mice.- C. elegans Methods to Study PTEN.

Erscheint lt. Verlag	11.12.2015
Reihe/Serie	Methods in Molecular Biology ; 1388
Zusatzinfo	17 Illustrations, color; 28 Illustrations, black and white; XI, 324 p. 45 illus., 17 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	C. elegans • ceRNAs • Diabetes • MicroRNAs • protein activity • protein localization • PTEN function in brain cancer • PTEN Hamartoma Tumor Syndrome • PTEN in diseased human specimens
ISBN-10	1-4939-3297-7 / 1493932977
ISBN-13	978-1-4939-3297-9 / 9781493932979
Zustand	Neuware