Baculovirus and Insect Cell Expression Protocols

Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior.- Introduction to Baculovirus Molecular Biology.- Baculovirus Transfer Vectors.- Recombinant Baculovirus Isolation.- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System.- Available Lepidopteran Insect Cell Lines.- Lepidopteran Insect Cell Line Isolation from Insect Tissue.- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines.- Routine Maintenance And Storage Of Lepidopteran Insect Cell.- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System.- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System.- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors.- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae.- Production Of Virus-Like Particles For Vaccination.- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells.- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression.- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression.- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression.- Introduction To The Use Of Baculoviruses As Biological Insecticides.- Baculovirus Insecticide Production In Insect Larvae.- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses.- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants.- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production.- Gene Silencing In Insect Cells Using Rnai.- Using the Baculovirus/Insect Cell System to Study Apoptosis.- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.