Gene Therapy for HIV and Chronic Infections (eBook)

Ben Berkhout is Head of the Laboratory of Experimental Virology at the Academic Medical Center (AMC) of the University of Amsterdam (the Netherlands), where he is Professor of Virology. Ben received his Ph.D. in 1986 at the University of Leiden on gene regulation in the RNA bacteriophage MS2. He did post-doctoral training at the Dana Farber Cancer Institute, Harvard Medical School (Boston) and the National Institutes of Health, Laboratory of Molecular Microbiology (Bethesda). He moved back to the Netherlands in 1991 to set up HIV-1 research at the University of Amsterdam. Ben has published over 440 scientific papers that were cited more than 14500 times, yielding an H-index of 62. He is currently editor of several journals (e.g. Retrovirology, Virus Research, Journal of Biomedical Science) and editorial board member of many more. His current research includes basic RNA studies (new RNAi mechanisms, structure and function of HIV-1 RNA) and applied research (RNAi-based gene therapy for HIV-AIDS, novel live-attenuated vaccine designs).Hildegund C. J. Ertl is a Professor at the Wistar Institute where she serves as the Director of the Wistar Vaccine Center. She is an Adjunct Professor in Pathology at the University of Pennsylvania and an Adjunct Professor in Pediatrics at the Children’s Hospital of Philadelphia. She obtained her MD in 1977 from the George-August University in Göttingen, Germany and did her postdoctoral studies at the John Curtin School in Canberra, Australia. She was an Assistant Professor at the Dana Farber Cancer Institute in Boston, MA and then moved as an Associate Professor to the Wistar Institute, Philadelphia, PA. Her research focuses on viral immunology with emphasis on development of viral vector vaccines and immunological consequences of virus-mediated gene transfer. She has over 220 publications and serves as an Editor on several journals (Current Opinion in Immunology, Current Opinion in Virology, Molecular Therapy, and others). Dr. Marc S. Weinberg is a molecular biologist and is currently Associate Professor at the University of the Witwatersrand and a Visiting Professor at the Scripps Research Institute, USA. His research focuses on the therapeutic functions of ribonucleic acid (RNA). He graduated with a PhD from The University of the Witwatersrand, South Africa in 2002 and was a postdoctoral fellow at City of Hope Medical Center, California, USA where studied fast-cleaving hammerhead ribozymes and RNA-based transcriptional modulators against HIV. Thereafter, his work turned to small RNAs that attenuate viral gene function and he was one of the first to show that small single-stranded RNAs can switch genes off directly using components of a nuclear RNA interference pathway. Dr. Weinberg has made important contributions to the development of novel RNA structures that are processed into safe and useful antiviral inhibitors, specifically against HIV and HBV. Lately, his work focuses on new ways to modulate non-coding RNA genes using cell-selected aptamers, CRISPR/Cas9 modulators and mobilization lentiviral vectors as delivery vehicles, with specific emphasis on how these approaches can be used against latent HIV infection. Dr. Weinberg has over 50 publications and holds 6 patents for RNA applications in biopharama and agriculture. He has also supervised 20 graduate students to completion. He is on the editorial board of Molecular Therapy Nucleic Acids, Journal of Biological Methods and the South African Journal of Science.

Chapter 1 - Gene Therapies for Hepatitis C Virus - Monique M.A. Verstegen, Qiuwei Pan and Luc J.W. van der LaanChapter 2 - Recent Advances in Use of Gene Therapy to Treat Hepatitis B Virus Infection - Kristie Bloom, Abdullah Ely and Patrick ArbuthnotChapter 3 - U1interference (U1i) for antiviral approaches - Lorea Blázquez and Puri FortesChapter 4 - Gene therapy strategies to block HIV-1 replication by RNA interference - Elena Herrera-Carrillo and Ben Berkhout Chapter 5 - HIV and Ribozymes - Robert J. Scarborough and Anne GatignolChapter 6 - Editing CCR5: a novel approach to HIV gene therapy - Tatjana Cornu, Claudio Mussolino, Kristie Bloom and Toni CathomenChapter 7 - Synthetic DNA approach to Cytomegalovirus vaccine/immune therapy - Stephan J. Wu, Daniel O. Villarreal, Devon J. Shedlock and David B. WeinerChapter 8 - Vector-mediated antibody gene transfer for infectious diseases - Bruce C. Schnepp and Philip R. JohnsonChapter 9 - HIV latency and the non-coding RNA therapeutic landscape - Sheena Saayman, Thomas C. Roberts, Kevin V. Morris and Marc S. WeinbergChapter 10 - C peptides as entry inhibitors for gene therapy - Lisa Egerer, Hans-Peter Kiem and Dorothee von LaerChapter 11 - Aptamer-siRNA chimeras for HIV - Mayumi Takahashi, John C. Burnett and John J. Rossi