Coronaviruses
Humana Press Inc. (Verlag)
978-1-4939-2437-0 (ISBN)
Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.
Coronaviruses: An Overview of Their Replication and Pathogenesis.- Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics.- Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test.- Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses.- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration.- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation.- Isolation and Propagation of Coronaviruses in Embryonated Eggs.- Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures.- Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo.- Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis.- Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes.- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues.- Identification of Protein Receptors for Coronaviruses by Mass Spectrometry.- Single Particle Tracking Assay to Study Coronavirus Membrane Fusion.- Studying Coronavirus-Host Protein Interactions.- A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions.- Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach.- Transcriptome Analysis ofFeline Infectious Peritonitis Virus (FIPV) Infection.- Quantification of Interferon Signaling in Avian Cells.- Studying the Dynamics of Coronavirus Replicative Structures.- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.
Reihe/Serie | Methods in Molecular Biology ; 1282 |
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Zusatzinfo | 27 Illustrations, color; 15 Illustrations, black and white; XI, 285 p. 42 illus., 27 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | coronovirology • titration techniques • Viral protein • virus-cell fusion • virus genomes • virus-host interactions • virus pathogenicity • virus replication |
ISBN-10 | 1-4939-2437-0 / 1493924370 |
ISBN-13 | 978-1-4939-2437-0 / 9781493924370 |
Zustand | Neuware |
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