Guide to Research Techniques in Neuroscience

Matt Carter is an Associate Professor of Biology at Williams College where he teaches courses in neuroscience and physiology. His research program focuses on how the brain regulates food intake and sleep and is funded by grants from the National Institutes of Health and National Science Foundation. In addition to primary scientific publications, he is also the author of Designing Science Presentations (Academic Press). He is a recipient of the Walter Gores Award for Excellence in Teaching from Stanford University and the Nelson Bushnell Prize for Excellence in Teaching and Writing from Williams College. Jennifer C. Shieh, PhD, is currently Program Director and SBIR (Small Business Innovation Research) Coordinator at the National Heart, Lung, and Blood Institute at the NIH. She co-authored the first edition of this book (Elsevier, 2009), and was awarded the Hans-Lukas Teuber Award for Outstanding Research and Academics in Brain and Cognitive Sciences, and the MIT Department of Brain and Cognitive Sciences Award for Outstanding Scholarship and BCS Contributions.

Chapter 1: Whole Brain Imaging Chapter 2: Animal Behavior Chapter 3: Stereotaxic Surgeries and In vivo techniquesChapter 4: ElectrophysiologyChapter 5: MicroscopyChapter 6: Visualizing Nervous System Structure Chapter 7: Visualizing Nervous System FunctionChapter 8: Manipulating Neural Activity Chapter 9: Identifying Genes and Proteins of InterestChapter 10: Molecular Cloning and Recombinant DNA TechnologyChapter 11: Gene Delivery StrategiesChapter 12: Making and using transgenic organismsChapter 13: Manipulating endogenous genes Chapter 14: Cell culture techniquesChapter 15: Biochemical assays and intracellular signaling