Biochemistry of Parasites (eBook)

Front Cover 1
The Biochemistry of Parasites 4
Copyright Page 5
Table of Contents 6
Introduction 10
BIOMEMBRANES SECTION 10
Part I: The Parasite Membrane 16
Chapter 1. ABSTRACT 18
Chapter 2. Structural, Chemical and Antigenic Properties of Surface Membranes Isolated from Leishmania Donovani 19
PELLICULAR MEMBRANE ISOLATION 19
FINE STRUCTURE OF ISOLATED MEMBRANES 20
PM CONSTITUENT SEPARATIONS 23
PM CARBOHYDRATE CONSTITUENTS 23
PM ENZYMES 27
DETERGENT SOLUBILIZATION OF PM 27
IMMUNOPRECIPITIN ANALYSES OF DETERGENT SOLUBILIZED PM 29
DISCUSSION, CONCLUSIONS AND SPECULATIONS 33
ACKNOWLEDGEMENT 37
REFERENCES 37
Chapter 3. ABSTRACT Phosphomonoesterase Activities at the Surface Membrane of Leishmania Donovani Promastigotes 38
Chapter 4. Phosphomonoesterase Activities at the Surface Membrane of Leishmania Donovani Promastigotes 39
MATERIALS AND METHODS 39
RESULTS 40
DISCUSSION 53
ACKNOWLEDGEMENT 54
REFERENCES 54
Chapter 5. ABSTRACT The Surface Membrane of Leishmania 56
Chapter 6. The Surface Membrane of Leishmania 57
LEISHMANIA CELL SURFACE 58
LONG TERM SECRETION STUDIES 66
SHORT TERM RELEASE OF LEISHMANIA MATERIAL TO THE MEDIUM 70
DISCUSSION 72
ACKNOWLEDGEMENT 73
REFERENCES 73
Chapter 7. ABSTRACT Alterations of Human Erythrocyte Membranes Due to Infection with Plasmodium Falciparum 76
Chapter 8. Alterations of Human Erythrocyte Membranes Due to Infection with Plasmodium Falciparum 77
REFERENCES 82
Chapter 9. ABSTRACT The Intestine of Ascaris Suum: Some Biochemical and Physiological Characteristics 84
Chapter 10. The Intestine of Ascaris Suum: Some Biochemical and Physiological Characteristics 85
Intracellular Na+ and K+ Activity 86
Glucose Induced Changes 87
Basement Membrane 88
CONCLUSIONS 91
ACKNOWLEDGEMENTS 91
REFERENCES 91
Chapter 11. ABSTRAC TInhibition of P. Falciparum Growth in vitro by Specific Inhibitors of Red Blood Cell Anion Transport 94
Chapter 12. Inhibition of P. Falciparum Growth in vitro by Specific Inhibitors of Red Blood Cell Anion Transport 95
LACTATE TRANSPORT ACROSS THE HUMAN ERYTHROCYTE MEMBRANE 97
RESULTS AND DISCUSSION 98
ACKNOWLEDGEMENTS 104
REFERENCES 104
Chapter 13. ABSTRACTS Externally Oriented Surface Membrane Cross-Reactive Carbohydrate Antigens in Leishmania Donovani and Trypanosoma Cruzi 106
Chapter 14. Immunological Analysis of Membrane Proteins of Leishmania Tropica Isolates of Different Virulence 106
Chapter 15. Lectin Analysis of the Variant Specific Antigen of T. Congolense 107
Chapter 16. Preparative Purification of the Variant Specific Glycoproteins of Trypanosoma Congolense 107
Chapter 17. Plasmodium Berghei: Serum Binding to Sporozoites 108
Part II: Adherence 110
Chapter 18. ABSTRACT Adherence of Pathogenic· Microorganisms to the Intestinal Tract 112
Chapter 19. Adherence of Pathogenic Microorganisms to the Intestinal Tract 113
CONCLUSION 124
ACKNOWLEDGEMENT 124
REFERENCES 125
Chapter 20. ABSTRACT Adherence of Mycoplasmas to Host Cell Membranes 126
Chapter 21. Adherence of Mycoplasmas to Host Cell Membranes 127
THE RECEPTORS 128
THE BINDING SITES 131
CONCLUDING REMARKS 134
REFERENCE 134
Chapter 22. ABSTRACT Amebiasis: Poorly Phagocytic Substrains of Entamoeba Histolytica Show Diminished Virulence 136
PARASITE METABOLISM SECTION 138
Introduction to the Metabolism Session 140
ACKNOWLEDGEMEN 142
REFERENCES 142
Part I: Protozoal Metabolism 144
Chapter 23. ABSTRACT The Proteases of Plasmodium: A Cathepsin D-like Enzyme from Plasmodium Lophurae 146
Chapter 24. The Proteases of Plasmodium: A Cathepsin D-like Enzyme from Plasmodium Lophurae 147
MATERIALS AND METHODS 149
RESULTS 150
DISCUSSION AND CONCLUSIONS 155
ACKNOWLEDGEMENT 157
REFERENCES 158
CHapter 25. ABSTRACT Regulation of Cyclic AMP-Metabolism in Leishmania Promastigotes and Amastigotes 160
Chapter 26. Regulation of Cyclic AMP-Metabolism in Leishmania Promastigotes and Amastigotes 161
CORRELATION BETWEEN CYCLIC AMP AND PROLIFERATION AND TRANSFORMATION OF LEISHMANIA CELLS 162
ADENYLATE CYCLASE 166
CYCLIC AMP PHOSPHODIESTERASE 168
CYCLIC AMP-BINDING PROTEINS 171
CONCLUDING REMARKS 173
ACKNOWLEDGEMENTS 174
REFERENCES 174
Chapter 27. ABSTRACTS The Mechanism of Aminopterin Resistance in Plasmodium Falciparum 178
Chapter 28. Isolation and Biochemical Characterization of Malaria Parasites (P. Vinckei) (P. Falciparum) 178
Chapter 29. A Sensitive Fluorescent Stain for Plasmodia Stages Obtained from a Percoll Gradient 179
Chapter 30. Inhibition of P. Falciparum Growth In Vitro by a Platelet Extract 179
Chapter 31. A New Method for the Measurement of the Influx of Chloroquine in Human Red Blood Cells 180
Chapter 32. Affinity Chromatographie Methods for the Purification of Leishmanial Excreted Factor 180
Chapter 33. A Possible Role for Leishmanial Excreted Factor (EF) in the Infective Process 181
Chapter 34. Kinetoplast DNA Sequences in Leishmania 181
Chapter 35. Toward the Primary Structure of Trypanosoma Brucei VSA's 182
Chapter 36. Enzymology of Energy Metabolism in Trypanosoma Cruzi Epimastigotes 182
Part II: Helminth Metabolism 184
Chapter 37. ABSTRACT Energy Generation in Parasitic Helminths 186
Chapter 38. Energy Generation in Parasitic Helminths 188
ACKNOWLEDGEMENT 197
REFERENCES 197
Chapter 39. ABSTRACT Liver Fibrosis in Schistosomiasis 200
Chapter 40. Liver Fibrosis in Schistosomiasis 201
SUBSTRATE REGULATION OF COLLAGEN SYNTHESIS 202
COLLAGENOLYSIS 205
CONNECTIONS BETWEEN SCHISTOSOME INFECTION AND COLLAGEN METABOLISM 207
ACKNOWLEDGEMENT 207
REFERENCES 207
Chapter 41. ABSTRACTS Pathways of Volatile Fatty Acid Production in Ascaris Lumbricoides with Particular Reference to the Synthesis of Succinyl-CoA 210
Chapter 42. The Aerobic Energy Metabolism of the Juvenile Liver Fluke, Fasciola Hepatica 210
Part III: Immuno- and Chemotherapy 212
Chapter 43. ABSTRACT Natural and Synthetic Immunostimulants and Transmethylase Inhibitors as Antiparasitic Agents in Animal Models 214
Chapter 44. Natural and Synthetic Immunostimulants and Transmethylase Inhibitors as Antiparasitic Agents in Animal Models 215
NATURAL AND SYNTHETIC IMMUNOSTIMULANTS 215
I. MDP and Derivatives 215
II. Trehalose 6,6'-diesters 218
III. Combined Immunostimulation 221
Conclusions 222
NATURAL AND SYNTHETIC TRANSMETHYLASE INHIBITORS 223
Conclusions 227
ACKNOWLEDGEMENTS 227
REFERENCES 227
Chapter 45. ABSTRACT Effect of Metrifonate and Diethylcarbamazine on Cholinesterase Activity from Onchocerca Volvulus 232
Author Index 234
Subject Index 236