Immuno-Gold Electron Microscopy in Virus Diagnosis and Research - Alexander D. Hyatt, Bryan Eaton

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Buch | Hardcover
464 Seiten
1992
Crc Press Inc (Verlag)
978-0-8493-6759-5 (ISBN)
399,95 inkl. MwSt
This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Alexander D. Hyatt, Bryan Eaton

Immuno-Gold Electron Microscopy for Virus Diagnosis: Immune Complexing (E.L. Palmer and M.L. Martin). Solid-Phase Immune Electron Microscopy of Virus Preparations (R.G. Milne). Gold Labeling in Negative Stain Immune Electron Microscopy (P.J.C. Dopping-Hepenstal and J.E. Beesley). The Grid-Cell-Culture Technique (A.D. Hyatt and B.T. Eaton). Use of Microspheres for Immune Scanning Electron Microscopy (J.P. Andersson, U.G. Andersson, and A. Sonnerborg). Immuno-Gold Electron Microscopy in Virus Research: The Colloidal Gold Probe, An Overview of Its Application in Viral Cytochemistry (S. Garzon and M. Bendayan). Detection of Viral and Cellular Structures by Post-Embedding Immunocytochemistry (G.N. Williams and P. Faulkner). Multiple Labeling of Thin Sections (M. Nielsen and L. Bastholm). Immuno-Gold Replica Technique for Study of Virus-Infected Cells (K. Mannweiler, H. Hohenberg, G. Rutter, and W. Bohn). Detection and Analysis of Viral Proteins by Immuno-Gold Labeling (K.G. Murti). Cryo-Sectioning (H.K. Hagler). Improvements of Immuno Dection Using Ultra-Small Gold Probes and Cryosections (J.L.M. Leunissen and P. van de Plas). Freeze Substitution in Virus Research: A Preview (H. Schwartz, H. Hohenberg, and B.M. Humbel). Ultrastructural In Situ Hybridization Using Immuno-Gold (G.L. Bassell and R.H. Singer). Use of Colloidal Gold and Silver Enhancement in Scanning Electron Microscopy (A.D. Hyatt and S.M. Brookes).

Erscheint lt. Verlag 10.11.1992
Zusatzinfo 16 Tables, black and white
Verlagsort Bosa Roca
Sprache englisch
Maße 156 x 234 mm
Gewicht 1020 g
Themenwelt Studium 2. Studienabschnitt (Klinik) Pathologie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
ISBN-10 0-8493-6759-X / 084936759X
ISBN-13 978-0-8493-6759-5 / 9780849367595
Zustand Neuware
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