Hepatitis C Protocols
Humana Press Inc. (Verlag)
978-1-4899-4348-4 (ISBN)
Detection of Anti-HCV in Serum.- Detection of Anti-GOR Antibodies in HCV Infection.- Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III.- Detection and Quantitation of HCV in Serum.- Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization.- Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR).- The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA.- Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology.- Quality Control of the Polymerase Chain Reaction.- Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays.- Detection of HCV in Liver Tissue.- Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR.- Quantification of HCV RNA in Liver Tissue by bDNA Assay.- HCV Genotypes.- Hepatitis C Virus.- Molecular Evolutionary Analysis.- Genotyping by Type-Specific Primers That Can Type HCV Types 1–6.- Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region.- Determination of HCV Genotypes by RFLP.- HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II.- Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region.- Determination of HCV Quasispecies by Cloning and Sequencing.- Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism.- Hepatitis C Virus Heteroduplex Tracking Assay.- Detection of HCV In Situ.- In Situ Detection of HCV.- In Situ Detection of Hepatitis C Viral Antigens.- In Situ Hybridization and the Detection and Localization of HCV RNA.- The In Situ Detection of PCR-Amplified Hepatitis C RNA.- lmmunoelectron Microscopic Characterization ofHCV.- Molecular Biologic Characterization.- Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones.- Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing.- Use of a Discistronic Vector for the Quantitation of HCV IRES Activity.- Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli.- Expression and Characterization of the HCV NS2 Protease.- Expression and Characterization of HCV NS3 Protease.- Expression and Characterization of the HCV NS3 Helicase Domain.- Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase).- Detection and Molecular Cloning of the Extreme 3?-End of HCV.- In Vitro Stability of Hepatitis C Virus RNA.- Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome.- HCV-Specific Immunologic Response.- Role of Immune Response in HCV.- Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC.- Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay.- Determination of HCV-Specific Bulk CD8+ Activity in Liver.- Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue.- Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization.- In Vitro Culture Model.- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification.- Strand-Specific rTth RT-PCR for the Analysis of HCV Replication.- Cell Culture Systems for the Detection of HCV Infection.- Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines.- Primary Human Hepatocyte Culture for the Study of HCV.- A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication.- Construction ofRecombinant Viral Vectors for the Expression of HCV Genes.- Construction of Recombinant Vaccinia Virus Expressing HCV Genes.- Construction of Recombinant Adeno-Associated Virus (AAV).- Production of Replication-Deficient Adenovirus Recombinants.- Generation of Recombinant Herpes Simplex Virus Ampiicons.- Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products.- In Vivo Models.- The Chimpanzee Model.- Liver Transplantation as a Model to Study Hepatitis C Virus Infection.
Reihe/Serie | Methods in Molecular Medicine ; 19 |
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Zusatzinfo | 171 Illustrations, black and white; XXI, 621 p. 171 illus. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 152 x 229 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
ISBN-10 | 1-4899-4348-X / 148994348X |
ISBN-13 | 978-1-4899-4348-4 / 9781489943484 |
Zustand | Neuware |
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