Resin Microscopy and On-Section Immunocytochemistry

I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Protocols for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Protocols for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I Examples of Typical Resin Embedding Regimes for Immunocytochemistry.- I.1 Solid Tissue, Pellets and Agar Blocks - Immersion Fixation.- I.2 Solid Tissue - Light Perfusion Fixation.- I.3 Solid Tissue - Very Light Perfusion Fixation.- I.4 Cryosubstitution.- I.5 Project Planner.- Appendix II List of Suppliers.- EM General.- EM Apparatus.- Electron Microscopes.- Flow Cytometry.- References.

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohistochemical laboratory. It extensively covers the field of onsection immunocytochemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling protocols ... . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. ... it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocytochemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)