The Nucleus

Cell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals.- Lysis Gradient Centrifugation – A Flexible Method for the Isolation of Nuclei from Primary Cells.- Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm.- A New Rapid Method for Isolating Nucleoli.- Sequential Recovery of Macromolecular Components of the Nucleolus.- Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus.- Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling.- Purification of Specific Chromatin Loci for Proteomic Analysis.- Chromatin Structure Analysis of Single Gene Molecules by Psoralen Crosslinking and Electron Microscopy.- Purification of Proteins on Newly Synthesized DNA Using iPOND.- Applying the Ribopuromycylation Method to Detect Nuclear Translation.- Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy.- A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox  Dynamics.- Determination of the Dissociation Constant of the NFkB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy.- Imaging and Quantification of Amyloid Fibrillation in the Cell Nucleus.- Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images.- Quantitative Analysis of Chromosome Localization in the Nucleus.