Regulatory Non-Coding RNAs
Humana Press Inc. (Verlag)
978-1-4939-1368-8 (ISBN)
Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.
Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs.- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.- A Biochemical Approach to Identify Direct MicroRNA Targets.- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs.- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets.- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells.- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells.- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins.- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale.- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization.- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells.- Locating RNAs In Situ with FISH-STIC Probes.- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides.- On Using Magnesium and Potassium Ions in RNA Experiments.- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.
Reihe/Serie | Methods in Molecular Biology ; 1206 |
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Zusatzinfo | 10 Illustrations, color; 29 Illustrations, black and white; X, 181 p. 39 illus., 10 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | Fluorescence in situ hybridization • long non-coding RNAs • MicroRNA targets • Non-coding transcriptome • Protein-RNA interactions • RNA-binding proteins • siRNAs |
ISBN-10 | 1-4939-1368-9 / 1493913689 |
ISBN-13 | 978-1-4939-1368-8 / 9781493913688 |
Zustand | Neuware |
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