Culture of Epithelial Cells

R. Ian Freshney gained a BSc (1960) and PhD (1964) from Glasgow University. He spent 1965 in Madison, Wisconsin, working on cell interaction and differentiation of embryonic mouse liver before returning to Glasgow to join the Beatson Institute for Cancer Research until 1981. At this time he transferred to Medical Oncology at Glasgow University, where he became a senior lecturer, teaching cell culture and tumor cell biology, with research interests in stromal interaction and differentiation in tumors. He is now an honorary senior research fellow at Glasgow University, focusing his attention on teaching, writing and editing in the field of cell culture. Mary G. Freshney gained considerable tissue culture experience, initially as a technician with Dr. John Paul, and laterally as a scientific assistant working on hematopoietic stem cells at the Beatson Institute, where she had the major responsibility for the management of the cell culture facility.

Contributors. Preface (R. Freshney & M. Freshney). Preface to the First Edition (R. Freshney). List of Abbreviations. Introduction (R. Freshney). Cell Interaction and Epithelial Differentiation (N. Maas Szabowski, et al.). The Epidermis (E. Parkinson & W. Yeudall). Culture of Human Mammary Epithelial Cells (M. Stampfer, et al.). Culture of Human Cervical Epithelial Cells (M. Stanley). Human Prostatic Epithelial Cells (D. Peehl). Human Oral Epithelium (R. Grafstrom). Normal Human Bronchial Epithelial Cell Culture (J. Wise & J. Lechner). Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells (L. Dobbs & R. Gonzalez). Isolation and Culture of Intestinal Epithelial Cells (C. Booth & J. O Shea). Isolation and Culture of Animal and Human Hepatocytes (C. Guguen Guillouzo). Culture of Human Urothelium (J. Southgate, et al.). Other Epithelial Cells (R. Freshney). List of Suppliers. Index.