RNA Mapping
Humana Press Inc. (Verlag)
978-1-4939-1061-8 (ISBN)
Full-Length Characterization Of Transcribed Genomic Regions.- Rapid Mapping Of RNA 3´and 5´Ends.- Single Nucleotide Mapping Of RNA 5´and 3´Ends.- RNA Secondary Structure Analysis.- Tertiary Structure Mapping Of Pri-Mirna17~92.- In Situ Hybridization Detection Of Micrornas Using LNA Oligonucleotides.- Quantification Of Micrornas By A Simple And Specific Qpcr Method.- RNA Isolation For Small RNA Next Generation Sequencing From Acellular Biofluids.- Sequencing Small RNA: Introduction and Data Analysis Fundamentals.- Measuring Expression Levels Of Small Regulatory RNA Molecules From Formalin-Fixed, Paraffin-Embedded Samples and Body Fluids.- Microrna Profiling In Plasma Or Serum Using Quantitative RT-Qpcr.- Microrna Profiling Of Exosomes Isolated From Biofluids And Conditioned Media.- Isolation Of Urinary Exosomes For RNA Biomarker Discovery Using A Simple, Fast, And Highly Scalable Method.- Identification Of Actively Translated Mrnas.- Mapping Of Internal Ribosome Entry Sites (IRES).- Mapping Of Protein Binding RNA Elements.- Purification Of RNA Binding Proteins .- De Novo Approach To Classify Protein-Coding And Non-Coding Transcripts By Utilizing Sequence Intrinsic Composition.- Computational Methods To Predict Long Non-Coding RNA Functions Based On Co-Expression Network.- Microrna Biogenesis - Dicing Assay.- Faster Experimental Validation Of Microrna Targets Using Cold Fusion Cloning And A Dual Firefly-Renilla Luciferase Reporter Assay.- Experimental Validation Of Predicted Mammalian Micrornas Of Mirtron Origin.- A Guide For Microrna Target Prediction And Analysis Using Web-Based Applications.- Tapping Microrna Regulation Networks Through Integrated Analysis Of Microrna-Mrna High-Throughput Profiles.- Mirwalk Database For Microrna-Target Interactions .- A Schematic Workflow For Collecting The Interaction Information Between Copy Number Variants And Micrornas Using Existing Resources.- SYBR Green And Taqman PCR Arrays: Expression Profile Of Genes RelevantTo A Pathway Or Disease State.- Comprehensive Meta-Analysis Of Microrna Expression Using A Robust Rank Aggregation Approach.
Reihe/Serie | Methods in Molecular Biology ; 1182 |
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Zusatzinfo | 53 Illustrations, color; 20 Illustrations, black and white; XI, 375 p. 73 illus., 53 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | cellular RNAs • diagnostic biomarkers • mapping of internal ribosome • microRNA targets prediction • novel RNA functions • quantitative real-time PCR • regulatory mRNA elements • RNA boundaries • RNA identification • RNA profiling • target RNAs • tertiary RNA • therapeutic targets |
ISBN-10 | 1-4939-1061-2 / 1493910612 |
ISBN-13 | 978-1-4939-1061-8 / 9781493910618 |
Zustand | Neuware |
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