An Atlas of Preimplantation Genetic Diagnosis

Foreword
Preface
Introduction
Section 1 Review of methods and experience in preimplantation genetic diagnosis: Normal and abnormal preimplantation development: selection of material for preimplantation genetic diagnosis: Oocytes at different stages of meiosis I. Oocytes at metaphase II and variations in the first polar body formation. Oocytes following fertilization and second polar body extrusion. Normally and abnormally cleaving embryos. Normal and abnormal blastocyst formation. Micromanipulation and biopsy of polar bodies and blastomeres: First polar body removal. Intracytoplasmic sperm injection after polar body removal. First and second polar body removal. Blastomere biopsy. Nuclear transfer techniques for visualization of chromosomes in polar bodies and blastomeres: Conversion of second polar body into metaphase chromosomes. Conversion of single blastomeres into metaphase chromosomes. Fluorescence in situ hybridization analysis of polar bodies and blastomeres: Preparations of polar bodies and blastomeres for fluorescence in situ hybridization analysis. Fluorescence in situ hybridization procedure. Fluorescent signal interpretation. PGD for chromosomal disorders by fluorescence in situ hybridization. Single-cell DNA analysis in polar bodies and blastomeres: Principles of PGD using first and second polar bodies. PCR analysis in single cells. Nested amplification. PGD for single-gene disorders. Future perspectives.
Section 2 Preimplantation genetic diagnosis illustrated. Index.
ISBN: 1-85070-760-X 174 pages (Hardback) Color illustrated
Due: August 2000 $149.95 / £98.00