Monoclonal Antibodies

Antigen Production for Monoclonal Antibody Generation.- Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications.- Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production.- Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion.- Generation of Rabbit Monoclonal Antibodies.- Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry.- Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker.- Design and Generation of Synthetic Antibody Libraries for Phage Display.- Selection and Screening using Antibody Phage Display Libraries.- Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening and Affinity Maturation.- Human B Cell Immortalization for Monoclonal Antibody Production.- Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice.- Cloning, Reformatting and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat or Hamster Hybridoma.- Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid.- Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography.- Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres.- Generation of Cell Lines for Monoclonal Antibody Production.- Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins.- Purification of Antibodies and Antibody Fragments using Capture Select Affinity Resins.- Reformatting of scFv antibodies into the scFv—Fc format and their Downstream Purification.- Antibody V and C Domain Sequence, Structure and Interaction Analysis with Special Reference to IMGT®.- Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface.- Determination of AntibodyStructures.- Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis.- Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides.- Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange and Peptide-Panning Techniques Combined with in silico Analysis.- Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen.- Epitope Mapping with Random Phage Display Library.- Epitope Mapping of Monoclonal and Polyclonal Antibodies using Bacterial Cell Surface Display.- Ion Exchange High Performance Liquid Chromatography (IEX-HPLC).- Size Exclusion High Performance Liquid Chromatography (SEC-HPLC).- N-glycosylation Characterization by Liquid Chromatography with Mass Spectrometry.- Fc-Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and their Functional Characterization.- Labeling and use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens.- Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization.- Antibody Array Generation and Use.