Functional Analysis of DNA and Chromatin

Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern.- Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions.- Identifying Different Types of Chromatin using Giemsa Staining.- Analysis of DNA Damage and Repair by Comet Fluorescence in situ Hybridization (Comet-FISH).- Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level.- Polarization Microscopy of Extended Chromatin Fibres.- DNA Labeling in Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software.- A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation.- Image Analysis of Chromatin Remodelling.- FISH Methods in Cytogenetic Studies.- Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker.- Atomic Force Microscopy for Analyzing Metaphase Chromosomes. Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns.- Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections.- Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies.- Visualization and Interpretation of Eukaryotic DNA Replication Intermediates in vivo by Electron Microscopy.- Combined Bi-dimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells.- Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection.- Methyl-combing—Single Molecule Analysis of DNA Methylation on Stretched DNA Fibers.- Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art.- Hydroxymethylated DNA Immunoprecipitation (hmeDIP).- Microscale Thermophoresis for the Assessment of Nuclear Protein Binding Affinities.- Analysis of Histone Post-translational Modificationsfrom Nucleolus-associated Chromatin by Mass Spectrometry.- Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation.- Chromatin Immunoprecipitation.- Analysis of Chromatin Composition of Repetitive Sequences: the ChIP-chop Assay.- Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae.- Deep Sequencing of Small Chromatin-Associated RNA – Isolation and Library Preparation.- Deep Sequencing of Small Chromatin-Associated RNA – Bioinformatic Analysis.