Gamete Assessment, Selection and Micromanipulation in ART

Zsolt Peter Nagy, MD, PhD, HCLD (ABB), EMB (ACE), Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, GA, USA Alex C. Varghese, PhD, Senior Embryologist, Montreal Reproductive Centre, Montreal, QC, Canada Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

​Part I: Human Oocyte Evaluation in IVF.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Part II: Sperm Evaluation and Selection.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in Andrology and IVF Laboratories.- Electrophoretic Sperm Separation.- Magnetic-Activated Cell Sorting of Human Spermatozoa.- Polscope-Based Sperm Selection.- Part III. Micromanipulators and Micromanipulation.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setting Up and Operation of Micromanipulators.- The Leica Microsystem IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Mechanism of Human Oocyte Activation during ICSI and Methodology for Overcoming Low or Failed Fertilization.- Livestock Production via Micromanipulation.- Assisted Hatching in IVF.- Part IV: Biopsy Procedures on Oocytes and Embryos.- Polar Body Biopsy.- Cleavage-Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Part V: Molecular Insights.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.